Transfer DNA - Wikipedia
网页The transfer DNA (abbreviated T-DNA) is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes (actually an Ri plasmid).
农杆菌的T-DNA是如何整合进植物基因组的？ - 知乎专栏
网页农杆菌菌株将单链形式的T-DNA（T链）和毒力效应蛋白（Vir）转移到植物细胞中。转移后，T链可能与Vir和植物蛋白形成复合物，然后通过细胞质运输并进入细胞核。
T-DNA - 搜狗百科
网页2024年6月12日 · T一DNA（Transfer DNA）即转移DNA，又名三螺旋DNA，是一种由三股ssDNA旋转螺旋形成的一种特殊脱氧核糖核苷酸结构。 T一DNA是Ti质粒上的一个片段。 利用农杆菌等微生物可将人工合成的目的基因片段通过T-DNA载体转移到受体植物的基因组中，是基因工程的重要技术手段。
Plant DNA Repair and Agrobacterium T−DNA Integration
网页2021年8月6日 · Agrobacterium species transfer DNA (T−DNA) to plant cells where it may integrate into plant chromosomes. The process of integration is thought to involve invasion and ligation of T-DNA, or its copying, into nicks or breaks in the host genome.
T-DNA integration in plants results from polymerase- - Nature
网页2016年10月31日 · Agrobacterium tumefaciens is a pathogenic bacterium, which transforms plants by transferring a discrete segment of its DNA, the T-DNA, to plant cells. The T-DNA then...
Regulation of gene editing using T-DNA concatenation - Nature
网页2023年8月31日 · Transfer DNA (T-DNA) originating from Agrobacterium can be integrated as a single copy or in complex concatenated forms 3, 4, but the mechanisms affecting final T-DNA structure remain...
The complex architecture and epigenomic impact of plant T-DNA …
网页2019年1月18日 · Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. Although these transfer mechanisms are well understood, detailed understanding of structure and epigenomic status of insertion events was limited by current technologies.
T-DNA–mediated transfer of Agrobacterium tumefaciens ... - Nature
网页2008年8月31日 · In nature, Agrobacterium transfers a defined segment of the tumor-inducing (Ti) plasmid (T-DNA) into the host, leading to the formation of crown gall tumors controlled by...
The Mechanism of T-DNA Integration: Some Major Unresolved Questions
网页2018年7月11日 · T-DNA integration requires the transfer of T-DNA from Agrobacterium into the plant cells. T-DNA transfer is a process related to bacterial conjugation (for review, see Lessl and Lanka 1994; Christie et al. 2005). Transfer begins inside the bacterium when T-DNA is separated from its parent plasmid, a Ti plasmid in natural strains or a binary ...
T-LOC: A comprehensive tool to localize and characterize T-DNA ...
网页2022年5月28日 · T-LOC has successfully recovered a diversified pattern of T-DNA integration: single-copy of ideal T-DNAs, inverted or tandem repeats of T-DNAs, T-DNA integration with small DNA fragment insertions, the integrated T-DNA in the repeated genomic region, truncated partial T-DNAs with or without selection marker, and a concatemer with multiple ...