The recent findings of Waldo et al. 1 demonstrate that the fusion of the green fluorescent protein (GFP) to insoluble proteins dramatically reduces its folding ability in prokaryotic cells.

A startup founded by former Meta researchers is creating an alternative evolutionary path using the most powerful ...

Plasmid vectors pET11a-link-NGFP and pMRBAD-link-CGFP and positive control vectors pET11a-Z-NGFP and pMRBAD-Z-CGFP are available upon request (Fig. 1) Figure 1: GFP-fragment reassembly vectors and ...

In this important manuscript, the authors provide evidence for this mechanism in two different mechanically gated channels. The experiments were carried out in the same membranes, but the evidence is ...

A team of researchers designed AI model capable of generating new proteins, trained on a simulation of 500 million years of ...

A team of AI researchers, biologists and evolutionary specialists at EvolutionaryScale and the Arc Institute, both in the U.S ...

Using this model, the researchers generated proteins with mutations that were predicted to lead to improved versions of GFP and a protein from adeno-associated virus (AAV), which is used to ...

This is a useful study that adds new data to how different DAG pools influence cellular signaling, and dissects how the enzyme Dip2 modulates the minor lipid signaling DAG pool, which is distinct from ...

Researchers discover how Zika virus and related viruses replicate in human cells, illuminating potential new vaccines.