In confocal microscopy, a pinhole is placed in front of the detector to exclude fluorescence emitted above and below the focal plane. However, this strategy reaches its limit when one wants to image ...

In confocal microscopy, the optical-sectioning ability is created by placement of a field stop, the so-called pinhole, in a conjugate image plane in front of a detector along the fluorescence beam ...

I built a 3D printed, scanning laser confocal microscope so I could collect 3D surface topology and profilometry data! The microscope stage is based on an OpenFlexure "Delta" stage, and the confocal ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Our basic Confocal Laser Scanning System includes an electronics control unit, an expandable dual PMT module, a 16-position pinhole wheel, a two-channel (488 nm & 642 nm) laser source, and the ...

The LSM 9 family with Airyscan 2 from ZEISS provides more options to enable the perfect balance of speed and resolution for today’s confocal-imaging needs. By combining the pinhole-plane imaging ...

Rather, for EL, the confocal pinhole diameter was leveraged to generate the spatial resolution by adjusting the pinhole ...

This 3D-printed confocal laser scanning microscope is a ... and a photodiode detector behind a pinhole. The detector feeds a homebrew trans-impedance amplifier that captures data at millions ...

A high-performance spinning disk confocal in an enclosed ... There are dual Nipkow disks with 50 or 25-micrometer lens pinhole arrays. The stage is motorized in X, Y, Z and coupled with Olympus’ ...