易基因｜不同DNA甲基转移酶（DNMT）在癌症发病机制中的作 …
DNA甲基转移酶（DNA methyltransferases，DNMT）在基因组DNA甲基化中起着至关重要的作用。. 在哺乳动物中，DNMT与某些元件一起调控胚胎和成体细胞的动态DNA甲基化模式。. 而DNMT异常功能通常是判断癌症的标志，包括抑癌基因（tumor suppressor genes，TSG）的整体 …
Knockdown of Dnmt1 and Dnmt3a gene expression disrupts
Knockdown of Dnmt genes using siRNAs was confirmed by measuring the targeted proteins using Western blot and immunofluorescence. Following knockdown of Dnmt1 and Dnmt3a in zygotes, the developmental competence and global DNA methylation levels were analyzed after 96 h in embryo cultures.
Inhibition of DNMT-1 alleviates ferroptosis through NCOA4
2021年9月29日 · 5-Aza-CdR is not a specific inhibitor for DNMT-1. To verify the effect of the inhibitor on damaged H9c2 cell cardiomyocytes, we compared the effect between siRNA of DNMT-1 and 5-aza-CdR.
DNMT1 is required to maintain CpG methylation and aberrant …
2002年12月23日 · We evaluated numerous antisense phosphorothioate oligo deoxynucleotides and small interfering RNAs (siRNA) spanning the DNMT1, DNMT3A and DNMT3B mRNA sequences that depleted mRNA of the target...
Consequences of combining siRNA-mediated DNA …
2015年6月6日 · SiRNA-mediated downregulation of each DNMT (DNMT1, 3A, and 3B) or concomitantly of the three together does not induce DNA demethylation, unlike the DNA demethylating agent DAC. First, the expression levels of DNMT1, 3A and 3B and their variant types were assessed in three blood cancer model cell lines, namely KG1, HL60 and Karpas299.
Double RNA Interference of DNMT3b and DNMT1 Enhances DNA …
2003年10月14日 · Small interfering RNAs (siRNAs) are newly identified molecules shown to silence genes via targeted mRNA degradation. In this study, we used specific siRNAs as a tool to probe the relationship between two DNA methyltransferase genes, DNMT3b and DNMT1, in the maintenance of DNA methylation patterns in the genome.
Multi-target siRNA based on DNMT3A/B homologous conserved …
2012年2月16日 · As the specific function of the DNMT3 subfamily in the initiation and development of prostate cancer remains elusive, it was decided to apply novel multi-target small interfering RNA (siRNA) targeting at the conservative region of human DNMT3 family proteins to the human prostate cancer cell line TSU-PR1, and then record and analyze subsequent c...
Knockdown of Dnmt1 and Dnmt3a gene expression disrupts …
2021年9月17日 · Knockdown of Dnmt genes using siRNAs was confirmed by measuring the targeted proteins using Western blot and immunofluorescence. Following knockdown of Dnmt1 and Dnmt3a in zygotes, the developmental competence and global DNA methylation levels were analyzed after 96 h in embryo cultures. Results.
Knockdown of DNA methyltransferase 3a alters gene expression …
To assess the role of DNMTs in these events, we investigated the effects of reduced DNMT expression on embryonic cardiomyocytes. siRNAs were used to knock down individual DNMT expression in primary cultures of mouse embryonic cardiomyocytes. Immunofluorescence staining was conducted to evaluate cell morphology.
Regulation of human RNA polymerase III transcription by DNMT1 …
2012年3月2日 · Indeed, both pharmacological inhibition of DNA methyltransferase (DNMT) activity and the forced diminution of DNMT-1, DNMT-3a, and DNMT-3b by siRNA targeting resulted in increased U6 levels in asynchronously growing MCF7 adenocarcinoma cells.
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