Development of Orthotopic Pancreatic Tumor Mouse Models
Xenograft mouse models of human pancreatic cancer including heterotopic and orthotopic have been widely used in preclinical studies for their comparatively low cost and rapid, predictable tumor growth.
Orthotopic Models | Charles River
Charles River offers orthotopic models across a broad range of histotypes to support your research needs. Orthotopic mouse models, involving tissue-specific tumor implantation, provide a disease-relevant tumor environment and better translation into the clinic.
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
Various breast cancer spontaneous metastasis mouse models have been established. Here, we report a simplified procedure to establish orthotopic transplanted breast cancer primary tumor and resultant spontaneous metastasis that mimic human breast cancer metastasis.
Orthotopic and heterotopic murine models of pancreatic cancer …
Here, we describe surgical techniques for orthotopic and heterotopic pancreatic ductal adenocarcinoma (PDAC) tumor implantation and characterize phenotypes based on implantation site. Mice (n =8 per group) were implanted with 10 4 cells in the pancreas or flank.
An orthotopic mouse model of gastric cancer invasion and
2018年1月16日 · To address this we have developed an orthotopic mouse model whereby gastric cancer cell lines are tagged with luciferase and injected into the subserosal layer of the stomach.
Orthotopic Mouse Models - Reaction Biology
The following syngeneic and xenograft orthotopic mouse models are available: Pancreatic tumor models, colon cancer models, breast cancer models, prostate cancer models, lung cancer models, skin cancer models such as melanoma, brain cancer models such as glioblastoma, and a kidney cancer model.
Orthotopic Mouse Models - Ichor Life Sciences
Particularly relevant for hard-to-treat cancers, such as pancreatic cancer and glioblastoma, an orthotopic mouse model offers a more accurate preclinical platform for drug development, because of its ability to mimic the physiological environment of human tumors to a greater extent.
Orthotopic mouse models for the preclinical and translational …
2014年11月11日 · In this article, we will summarize results on preclinical testing of selective and nonselective single compounds in orthotopic mouse models based on validated human thyroid cancer cell lines harboring the BRAF V600E mutation or with wild-type BRAF.
Establishment of an orthotopic glioblastoma mouse model for
Different in vitro models are currently used, including commercial cell lines, patient-derived cell lines, organoids, as well as in vivo models, being orthotopic xenografts the most used ones. In this chapter, we describe a standard protocol for the intracranial inoculation of glioblastoma cells in immunodeficient mice, and how to follow up the ...
Development of a Novel Orthotopic Gastric Cancer Mouse Model
2021年1月4日 · The present study aims to develop a new method that enables transplantation of various types of materials derived from gastric tumors into the mouse stomach in order to overcome the existing shortcomings of orthotopic methods and also to assess the clinical reliability of the orthotopic gastric cancer model.
A syngeneic MC38 orthotopic mouse model of colorectal cancer …
Orthotopic tumors, grown from the organ corresponding with the cancer type, provide a more translational approach to study disease progression and drug efficacy. Utilization of a syngeneic mouse model allows for a complete immune landscape, key for adaptive immunotherapy studies.
Development of orthotopic tumour models using ultrasound …
2019年7月9日 · In this study, we have developed orthotopic colorectal cancer liver metastases (CRCLM) and primary cholangiocarcinoma (CCA) models in BALB/c nude mice using minimally invasive...
Validation of an orthotopic non-small cell lung cancer mouse model ...
2023年4月13日 · We proposed and reported on and validated the efficacy of an orthotopic mouse model of NSCLC featuring H1299 NSCLC cell line, in both the left and right lung, using a non-invasive computed tomography (CT) imaging of tumor quantification.
A syngeneic MC38 orthotopic mouse model of colorectal cancer …
2022年9月28日 · Utilization of a syngeneic mouse model allows for a complete immune landscape, key for adaptive immunotherapy studies. MC38 and CT26 cells are commonly used murine colorectal cancer cell lines with clinically relevant mutations.
Orthotopic gastric cancer mouse model identifies trajectory of ...
2024年12月17日 · Orthotopic gastric cancer mouse model identifies trajectory of lymphatic metastasis Huafeng Fu, 1 Qinbo Cai, 2 Zhijun Zhou, 3 Yulong He, 1 Min Li, 3 and DongJie Yang 1
Generation of an orthotopic mouse model to study colorectal …
2021年12月17日 · Compared to existing protocols, this protocol is mainly to improve the incidence of metastasis. The major advantage of this model is that it mimics the process of clinical CRC metastasis. Thus, it can be used to study different stages of CRC progression and to identify molecular markers or therapeutic targets.
Orthotopic mouse model of colorectal cancer - PubMed
Orthotopic mouse models of colorectal cancer, which feature cancer cells growing in their natural location, replicate human disease with high fidelity. Two techniques can be used to establish this model.
Orthotopic and metastatic tumour models in preclinical cancer …
2024年5月1日 · Important developments in orthotopic models, such as the increasing use of early passage patient material (PDXs, organoids) and humanised mouse models are discussed, as these approaches have the potential to increase the predictive value of preclinical studies, and ultimately improve the success rate of anticancer drugs in clinical trials.
Orthotopic Mouse Models of Urinary Bladder Cancer - PMC
Here, we summarize and compare two representative methods used to create the syngeneic mouse models required for immunological research. In this study, we utilized six-week-old female C3H/HeNCrl mice and the mouse bladder tumor cell line MBT-2.
Orthotopic Mouse Model of Colorectal Cancer - PMC
Orthotopic mouse models of colorectal cancer, which feature cancer cells growing in their natural location, replicate human disease with high fidelity. The two techniques to establish this model have unique advantages and disadvantages.
Orthotopic meningioma rat model exhibits morphological and ...
2024年12月30日 · Nakano, T. et al. Eribulin prolongs survival in an orthotopic xenograft mouse model of malignant meningioma. Cancer Sci. 113 (2), 697–708 (2022). Article CAS PubMed Google Scholar
A Method for Orthotopic Transplantation of Lung Cancer in Mice
Here, we present an orthotopic mouse model of lung cancer that utilizes direct injection of cancer cells into the lung parenchyma that allows many potential studies including interactions of lung fibroblast Hedgehog pathway activity and tumor epithelia.
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