Organoids - Nature Reviews Methods Primers
2022年12月1日 · Organoids are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function of the corresponding in vivo tissue.
Organoid culture media formulated with growth factors of …
2019年4月17日 · We detail the workflow for Gremlin 1 and R-spondin 1 expression, purification, quantification of cellular activity, quality control and use in media formulated for culturing organoids derived from...
Scalable production of uniform and mature organoids in a 3D ...
2024年10月31日 · Using UniMat, we demonstrate the scalable generation of kidney organoids with enhanced uniformity in both structure and function compared to conventional methods.
Organoids - PMC
Organoid growth is a process of initial cell aggregation, proliferation, migration and differentiation. When assessing whether the organoids have been successfully established, it is critical to first determine whether the organoids contain the desired cell types, and the degree to which the organoids accurately mimic the functions of the ...
Organoids: Modeling Development and the Stem Cell Niche in …
2016年9月26日 · Organoid technology allows the establishment of long-term stem cell-based organotypic cultures in the absence of feeder cells by supplementing the culture medium with well-defined stem cell niche factors. A variety of different organoid culture methods have been described over the past years.
Organoids: development and applications in disease models, drug ...
Organoids are miniature, highly accurate representations of organs that capture the structure and unique functions of specific organs. Although the field of organoids has experienced exponential growth, driven by advances in artificial intelligence, ...
Recent advances in organoid development and ... - ScienceDirect
2021年4月1日 · An improved understanding of stem cell niches, organogenesis, and disease models has paved the way for developing a three-dimensional (3D) organoid culture system. Organoid cultures can be derived from primary tissues (single cells or tissue subunits), adult stem cells (ASCs), induced pluripotent stem cells (iPSCs), or embryonic stem cells (ESCs).
Growth of Epithelial Organoids in a Defined Hydrogel
2018年9月10日 · Epithelial organoids are simplified models of organs grown in vitro from embryonic and adult stem cells. They are widely used to study organ development and disease, and enable drug screening in patient-derived primary tissues.
Recent advances in organoid engineering: A comprehensive review
2022年12月1日 · Organoids play a vital role in tissue and organ biology. Organoids have an ability to mimic the actual physiological conditions. Current organoid technology integrates bioengineering concepts. Recent trends and future perspectives of the organoids are …
Next-Generation Surrogate Wnts Support Organoid Growth …
2020年11月5日 · NGS Wnts are superior to Wnt3a conditioned media in organoid expansion and single-cell organoid outgrowth. Administration of Fzd subtype-specific NGS Wnt in vivo reveals that adult intestinal crypt proliferation can be promoted by agonism of Fzd5 and/or Fzd8 receptors, while a broad spectrum of Fzd receptors can induce liver zonation.