Advances in lysosomal escape mechanisms for ... - ScienceDirect
2024年10月15日 · A schematic overview of three main strategies used for overcoming lysosomal barriers: (A) strategies to promote endosomal/lysosomal escape, (B) strategies for directly crossing the cell membrane without entering endosomes or lysosomes, and (C) strategies to change the pathway to evade lysosomes and avoid degradation.
纳米载体逃逸溶酶体机制及其调控的研究进展 - 仁和软件
2016年11月8日 · 而纳米载体的转运效率很大程度上取决于其逃逸溶酶体(lysosome)的能力，即纳米载体进入细胞后，经膜泡包被并传递进入溶酶体，成功逃离至细胞质，保护其负载物避免被溶酶体内的多种消化酶降解。
Lysosomal escaped protein nanocarriers for nuclear-targeted …
2021年3月26日 · HA peptide can destroy lysosome membrane, make siRNA/NLS escape lysosome, and enter the nucleus under the action of NLS, resulting in efficient gene silencing effect. This kind of cancer treatment strategy based on Apn nanocage shows high biocompatibility and unique lysosome escape property, which significantly improves the drug delivery and ...
Advanced Strategies for Overcoming Endosomal/Lysosomal …
A schematic overview of 3 main strategies used for overcoming lysosomal barriers. (A) Strategies to promote endosomal/lysosomal escape. (B) Strategies for directly crossing the cell membrane without entering endosomes or lysosomes. (C) Strategies to change the pathway to evade lysosomes and avoid degradation.
Escaping the endosome: assessing cellular trafficking mechanisms of …
2021年7月10日 · Each compartment in the pathway is marked by characteristic proteins, such as early endosome antigen 1 (EEA1), lysosomal-associated membrane protein 1 (LAMP1), and Rab family GTPases. In this review, we focus on quantitative and qualitative methods to assess the intracellular fate of non-viral vehicles.
Endosomal escape: A bottleneck for LNP-mediated therapeutics
The release of the payload prior to lysosomal maturation is a crucial stage for efficient delivery and is known as endosomal escape. This process is inefficient and is considered a bottleneck in this field (17, 18).
Linkage between endosomal escape of LNP-mRNA and loading …
2019年9月24日 · Endosomal escape of LNP-mRNA is dependent on the molar ratio between ionizable lipids and mRNA nucleotides. Our results show that fractions of ionizable lipids and mRNA (1:1 molar ratio of hEPO...
Imaging small molecule-induced endosomal escape of siRNA
2020年4月14日 · Here, we use live-cell microscopy and cytosolic galectin-9 as a sensor of membrane damage, to probe fundamental properties of endosomal escape of cholesterol-conjugated siRNA induced by endosome...
Nanomechanical action opens endo-lysosomal compartments
2023年10月20日 · Endo-lysosomal escape is a highly inefficient process. Here the authors present a lipid-based nanoscale molecular machine that achieves efficient cytosolic transport of biologics by...
Achieving Endo/Lysosomal Escape Using Smart Nanosystems for …
2024年7月1日 · Overcoming the endosomal/lysosomal barrier is essential for successful nanodrug delivery in the treatment of various diseases. This article outlines the significance of endosomal entrapment, emphasizing its role as a barrier to drug/biomolecule delivery and the need for “smart” nanosystems to overcome it.