Chromatographic analysis of α-tocopherol and related …
2001年11月23日 · The new method consisted of extraction of α-tocopherol from biological samples, purification by semipreparative HPLC, conversion to α-tocopherol methylesters, separation by HPLC into five peaks and GC analysis of one of the peaks formed by four isomers.
Simple GC-FID method development and validation for …
2007年4月10日 · The present study describes the novel GC-FID method for determination of α-tocopherol (vitamin E) without derivatization in human plasma. The proposed method is simpler than HPLC methods and more reliable than spectrometric methods and easily applied to the analysis of large numbers of human plasma.
GC–MS and LC–MS approaches for determination of tocopherols …
2016年8月5日 · In this paper we present current analytical approaches in tocopherols and tocotrienols determination in biological and food matrices with the use of chromatographic techniques, especially gas chromatography (GC) and high performance liquid chromatography (HPLC) coupled with mass spectrometry.
(PDF) Simple GC-FID method development and validation
2007年5月1日 · A rapid, high-selectivity method with subfemtomole sensitivity is reported for quantification of alpha-tocopherol in plasma-based gas chromatography/tandem mass spectrometry (GC/MS/MS) using...
Quantitative Subfemtomole Analysis of α-Tocopherol and …
A rapid, high-selectivity method with subfemtomole sensitivity is reported for quantification of α-tocopherol in plasma-based gas chromatography/tandem mass spectrometry (GC/MS/MS) using a tabletop quadrupole ion trap mass spectrometer.
A gas chromatographic method was applied by using the GC-FID tehnique for the determination of α-tocopherol acetate in an anti-aging properly developed cosmetic formulation. A simple methanol:acetonitrile organic solvent mixture (50:50v/v) was required for sample preparation and the separation of the studied
report discusses the use of a gas chromatograph coupled to a bench top ion trap mass spectrometer running in single ion monitoring mode. Figures 1 and 2 on page 1 show the results of gamma- and alpha-tocopherol in a pooled serum sample that are at “low baseline” levels (i.e. from individuals not taking any alpha-tocopherol containing drugs).
Gas chromatography-mass spectrometry (GC-MS) chromatogram …
The GC-MS chromatogram was obtained by directly injecting a standard of α-tocopherol, α-tocopheryl acetate, phylloquinone, and menaquinone-4 at a concentration level of 50 µg mL −1 using the ...
Gas chromatography-mass spectrometry microanalysis of alpha …
2015年6月15日 · Alpha-TOH correlated with total cholesterol (r = 0.617, p < 0.0001) and triglycerides (r = 0.420, p < 0.0001) while γ-TOH correlated modestly with total cholesterol (r = 0.213, p < 0.0001) but not with triglycerides. γ-TOH, but not α-TOH, was significantly lower in smokers than in non-smokers (0.72 ± 0.50 vs. 0.56 ± 0.37, μM, mean ± SD ...
Gas chromatography–mass spectrometry microanalysis of alpha …
2015年6月15日 · GC–MS analysis of alpha- and gamma-tocopherol. SIM chromatograms of representative plasma samples are displayed for α-tocopherol (A, upper panel) and γ-tocopherol (B, lower panel).
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