Cells of the adult human heart | Nature
2020年9月24日 · NC5 expresses LGR5, a Wnt signalling, G-protein-coupled receptor and stem-cell marker that promotes cardiomyocyte differentiation in the outflow tract 54, an arrhythmogenic area 55.
Generation of mature compact ventricular cardiomyocytes from human ...
2021年5月26日 · The demonstration that the mature cells upregulate LDLR and that its expression can be measured by flow cytometry provides a second surface marker for monitoring cardiomyocyte maturation in the...
Cell proliferation fate mapping reveals regional cardiomyocyte cell ...
2021年10月1日 · This trend was confirmed by manually counting of dissociated cardiomyocytes (Fig. 1f) as well as by immunostaining of heart sections with antibodies against GFP, the cardiomyocyte marker TNNI3 ...
Defining Cardiomyocyte Repolarization Response to …
2024年10月8日 · The monolayer‐differentiated cells on day 28 were examined using immunofluorescence staining to assess the protein expression levels of the cardiomyocyte‐associated markers troponin T (TNNT2), sarcomere‐associated marker α‐actinin (ACTN2), and ventricular cardiomyocyte subtype‐associated marker ventricular myosin light chain MLC‐2v ...
Cardiomyocyte Maturation: New Phase in Development
2020年4月10日 · Here, we review the major hallmarks of ventricular cardiomyocyte maturation and summarize key regulatory mechanisms that promote and coordinate these cellular events.
JAK2 as a surface marker for enrichment of human pluripotent …
2023年12月13日 · Our study lays the groundwork for the identification of cardiac subtype surface markers that allow purification of cardiomyocyte sub-populations. Our findings suggest that JAK2 can be employed as a cell surface marker for enrichment of …
Heart Regeneration by Endogenous Stem Cells and Cardiomyocyte ...
2020年7月21日 · Whereas the studies using isotype incorporation, marker staining, or genetic fate mapping approaches consistently revealed the limited self-renewal capacity of cardiomyocytes, there are obvious discrepancies in the magnitude of cardiomyocyte generation measured from these different methods.
Cardiomyocyte maturation: advances in knowledge and …
Postnatal developmental changes in cardiomyocytes are remarkable. The cells withdraw from the cell cycle, typically undergoing one more round of DNA synthesis without cell division, generating large, tetraploid nuclei (in humans) or binucleated myocytes (in rodents) 6, 7.
Measuring cardiomyocyte cell-cycle activity and proliferation in …
2022年3月17日 · Evidence for human cardiomyocyte cell cycling has been obtained from studies of human heart tissue by direct assessment of cell-cycle markers on histological sections and by calculating cardiomyocyte nuclear turnover in myocardial samples from individuals exposed to 14 C radiation with nuclear bomb tests .
A method for differentiating human induced pluripotent stem …
2020年10月28日 · Several approaches to enrich the cardiomyocyte population have been described including genetic-modification (by the expression of MYH6), using surface protein as a specific marker for ...