AOM/DSS Model of Colitis-Associated Cancer - PMC - PubMed …
Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …
Inducible mouse models of colon cancer for the analysis of ... - Nature
Dec 14, 2020 · Bissahoyo, A. et al. Azoxymethane is a genetic background-dependent colorectal tumor initiator and promoter in mice: effects of dose, route, and diet. Toxicol. Sci. 88 , 340–345 (2005).
AOM/DSS Model of Colitis-Associated Cancer - PubMed
Chemical models, in particular, have enabled fast and efficient analysis of genetic and environmental modulators of CAC without the added requirement of time-intensive genetic crossings. Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer.
Protocol for colitis-associated colorectal cancer murine model …
Mar 17, 2023 · Here, we describe a step-by-step protocol for the construction of colitis-associated CRC murine model by sequential utilization of azoxymethane and dextran sulfate sodium. We also detail steps to determine the degree of murine intestinal inflammation and to generate colorectum Swiss roll for further histopathological analyses.
Murine models of colorectal cancer: the azoxymethane (AOM
Oct 31, 2023 · Results: Existing murine models of CRC include spontaneous, genetically engineered, transplantation, and chemically induced models. For the study of colitis-associated cancer (CAC), the AOM/DSS model is predominantly used.
An inducible mouse model of colon carcinogenesis for the …
Aug 9, 2007 · Here, we provide a highly reliable protocol for an experimental mouse model to study the development of colon cancers. It is based on the mutagenic agent azoxymethane (AOM), which exerts...
Induction of colorectal carcinogenesis in the C57BL/6J and A/J …
Jul 27, 2021 · The Azoxymethane/Dextran sulfate sodium (AOM/DSS) model is a widely used, robust initiation-promotion model for chemical induction of colitis-associated CRC in rodents. However, the dosage of chemicals, treatment regimens and outcome measures vary greatly among studies employing this model.
Azoxymethane/Dextran Sodium Sulfate (AOM/DSS) Model of Colorectal Cancer
Jan 19, 2024 · Among them, we used azoxymethane (AOM), an alkylating agent which induces DNA-reactive adducts, combined with the inflammatory agent dextran sodium sulfate (DSS) to obtain a mouse model of inflammation-driven sporadic colorectal cancer (CRC) .
Modeling Colitis-Associated Cancer with Azoxymethane (AOM) …
Here, we demonstrate a protocol for inflammation-driven colonic tumorigenesis in mice utilizing a single injection of AOM followed by three seven-day cycles of DSS over a 10 week period.
AOM/DSS Model of Colitis-Associated Cancer | SpringerLink
Jun 1, 2016 · Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by repeated cycles of colitis [12 – 15].
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