AOM/DSS Model of Colitis-Associated Cancer - PMC - PubMed …
Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …
Murine models of colorectal cancer: the azoxymethane (AOM
2023年10月31日 · Results: Existing murine models of CRC include spontaneous, genetically engineered, transplantation, and chemically induced models. For the study of colitis-associated cancer (CAC), the AOM/DSS model is predominantly used.
Protocol for colitis-associated colorectal cancer murine model …
2023年3月17日 · Here, we describe a step-by-step protocol for the construction of colitis-associated CRC murine model by sequential utilization of azoxymethane and dextran sulfate sodium. We also detail steps to determine the degree of murine intestinal inflammation and to generate colorectum Swiss roll for further histopathological analyses.
Inducible mouse models of colon cancer for the analysis of ... - Nature
2020年12月14日 · Bissahoyo, A. et al. Azoxymethane is a genetic background-dependent colorectal tumor initiator and promoter in mice: effects of dose, route, and diet. Toxicol. Sci. 88 , 340–345 (2005).
An inducible mouse model of colon carcinogenesis for the …
2007年8月9日 · Here, we provide a highly reliable protocol for an experimental mouse model to study the development of colon cancers. It is based on the mutagenic agent azoxymethane (AOM), which exerts...
AOM/DSS Model of Colitis-Associated Cancer - PubMed
Chemical models, in particular, have enabled fast and efficient analysis of genetic and environmental modulators of CAC without the added requirement of time-intensive genetic crossings. Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer.
Induction of colorectal carcinogenesis in the C57BL/6J and A/J …
2021年7月27日 · The Azoxymethane/Dextran sulfate sodium (AOM/DSS) model is a widely used, robust initiation-promotion model for chemical induction of colitis-associated CRC in rodents. However, the dosage of chemicals, treatment regimens and outcome measures vary greatly among studies employing this model.
Azoxymethane/Dextran Sodium Sulfate (AOM/DSS) Model of Colorectal Cancer
2024年1月19日 · Among them, we used azoxymethane (AOM), an alkylating agent which induces DNA-reactive adducts, combined with the inflammatory agent dextran sodium sulfate (DSS) to obtain a mouse model of inflammation-driven sporadic colorectal cancer (CRC) .
Modeling Colitis-Associated Cancer with Azoxymethane (AOM) …
Here, we demonstrate a protocol for inflammation-driven colonic tumorigenesis in mice utilizing a single injection of AOM followed by three seven-day cycles of DSS over a 10 week period.
Genomic variants in mouse model induced by azoxymethane …
2017年2月7日 · Mouse model induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) is generally accepted as an ideal object to study on the carcinogenesis mechanisms of human colorectal cancer...