AOM/DSS Model of Colitis-Associated Cancer - PMC - PubMed …
Here we outline the application of the Azoxymethane (AOM)/Dextran sodium sulfate (DSS) model of CAC. The AOM/DSS model is a powerful, reproducible, and relatively inexpensive initiation-promotion model that utilizes chemical induction of DNA damage followed by …
AOM/DSS Model of Colitis-Associated Cancer - PubMed
Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer. Keywords: AOM; Colitis-associated cancer; Colon cancer; DSS; Inflammatory carcinogenesis. Our understanding of colitis-associated carcinoma (CAC) has benefited substantially from mouse models that faithfully recapitulate human CAC.
Inducible mouse models of colon cancer for the analysis of ... - Nature
2020年12月14日 · This protocol updates our protocol published in 2007, which provided an azoxymethane (AOM)-based setup for investigations into sporadic (Step 5A) and, when combined with dextran sodium sulfate...
Protocol for colitis-associated colorectal cancer murine model …
2023年3月17日 · Here we describe the details of the inflammation-dependent colitis-associated CRC model through a combined induction of AOM and DSS, in which genotoxic agent AOM induces O 6-methylguanine adducts resulting in G→A transitions in DNA, while discontinued DSS administration triggers colitis-dependent neoplasia leading to inflammatory ...
Here we describe the Azoxymethane (AOM)/ Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer. 1. Introduction. Colorectal cancer (CRC) is the fourth most common cancer in the world (1). It is well established that colitis predisposes individuals to colorectal tumorigenesis (2–4).
Molecular mechanisms in colitis-associated colorectal cancer
2023年10月26日 · In this Review, we discuss recent genetic, epigenetic, and environmental findings related to CAC pathogenesis in the past five years, with a focus on unbiased, high-resolution genetic profiling of...
Mouse Models of Colitis-Associated Colon Cancer - PubMed
AOM-DSS-induced colitis-associated colon cancer in mice is the most widely used and accepted model that can recapitulate the human IBD-associated colon cancer. Here, we have provided detailed protocols of this mouse model of experimentally induced chronic intestinal inflammation-associated colon cancer.
The AOM/DSS murine model for the study of colon …
2011年10月1日 · In this article we highlight the histopathological and molecular features and describe the principal genetic and epigenetic alterations and inflammatory pathways involved in carcinogenesis in AOM/DSS-treated mice; we also present a general overview of recent experimental applications and preclinical testing of novel therapeutics in the AOM/DSS ...
Murine models of colorectal cancer: the azoxymethane (AOM
2023年10月31日 · Results: Existing murine models of CRC include spontaneous, genetically engineered, transplantation, and chemically induced models. For the study of colitis-associated cancer (CAC), the AOM/DSS model is predominantly used.
AOM/DSS Model of Colitis-Associated Cancer | SpringerLink
2016年6月1日 · Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer. Colorectal cancer (CRC) is the fourth most common cancer in the world [1]. It is well established that colitis predisposes individuals to colorectal tumorigenesis [2 – 4].