Role of adaptin protein complexes in intracellular trafficking and ...
AP1 and AP2 are the more ‘classic’ APs that interact with clathrin in the TGN (AP1) or plasma membrane (AP2) to form clathrin-coated vesicles and control intracellular trafficking in the TGN and endocytosis, respectively. The location and function of AP3 are less well defined compared to those of AP1 and AP2.
Conformational regulation of AP1 and AP2 clathrin adaptor …
2019年7月17日 · Heterotetrameric clathrin adaptor protein complexes (APs) orchestrate the formation of coated vesicles for transport among organelles of the cell periphery. AP1 binds membranes enriched for phosphatidylinositol 4-phosphate, such as the trans Golgi network, while AP2 associates with phosphatidylinositol 4,5-bisphosphate of the plasma membrane.
Full article: Role of adaptin protein complexes in intracellular ...
AP1 and AP2 are the more ‘classic’ APs that interact with clathrin in the TGN (AP1) or plasma membrane (AP2) to form clathrin-coated vesicles and control intracellular trafficking in the TGN and endocytosis, respectively. The location and function of AP3 are less well defined compared to those of AP1 and AP2.
Architecture of the AP2/clathrin coat on the membranes of …
2020年7月22日 · Adaptor protein complex (AP2) is the key adaptor in CME. Crystallography has shown AP2 to adopt a range of conformations. Here, we used cryo–electron microscopy, tomography, and subtomogram averaging to determine structures, interactions, and arrangements of clathrin and AP2 at the key steps of coat assembly, from AP2 in solution to membrane ...
Life of a clathrin coat: insights from clathrin and AP structures
2006年1月1日 · The first clathrin adaptors to be identified were the protein-cargo-binding and phospholipid-binding heterotetrameric adaptor protein complexes (APs) AP1 and AP2, and, to this day, they...
AAGAB is an assembly chaperone regulating AP1 and AP2 …
2021年10月1日 · AAGAB promotes AP1 assembly by binding and stabilizing the γ and σ subunits of AP1, and its mutation abolishes AP1 assembly and disrupts AP1-mediated cargo trafficking. Comparative proteomic analyses indicate that AAGAB mutation massively alters surface protein homeostasis, and its loss-of-function phenotypes reflect the synergistic effects ...
Adaptor protein complexes and disease at a glance
2019年10月21日 · Adaptor protein (AP) complexes are heterotetramers that select cargo for inclusion into transport vesicles. Five AP complexes (AP-1 to AP-5) have been described, each with a distinct localisation and function.
New directions for the clathrin adaptor AP-1 in cell ... - ScienceDirect
2022年6月1日 · Several new studies have revealed unexpected roles for AP-1 in polarized protein localization. Although AP-1 is best known for its roles in traffic between the TGN and endosomes, it is well-established that it also functions in traffic to the plasma membrane in epithelial cells.
Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2 ...
2021年4月13日 · The AP1/σ1B ko induced secondary phenotype in AP2 CCV pathways enables us to investigate physiological mechanisms regulating the AP2 CCV life cycle. CCV coat protein ko and protein domain overexpression studies revealed coat protein interactions and functions in …
Conformational regulation of AP1 and AP2 clathrin adaptor …
Heterotetrameric clathrin adaptor protein complexes (APs) orchestrate the formation of coated vesicles for transport among organelles of the cell periphery. AP1 binds membranes enriched for phosphatidylinositol 4-phosphate, such as the trans Golgi network, while AP2 associates with phosphatidylinosi …