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Perform the following extraction twice: add 1 ml of water-saturated diethyl ether, vortex the tube, and dispose of the upper (solvent) phase. Ensure that the diethyl ether is water-saturated ...

The expression vector and the preparation of HVJ–liposome complexes. Rats were anesthetized with diethyl–ether, and their gluteal muscles were exposed. The HVJ liposome solution (20 mg or 40 ...